Dr. Ariel Orellana

Dr. Ariel Orellana Lopez

Profesor Titular

Bioquímico, Universidad de Concepción, Concepción, Chile

Doctor en Ciencias Biológicas, Biología Celular y Molecular, P. Universidad Católica de Chile

Área de especialización: Síntesis de Polisacáridos de pared celular en el Golgi: Contribución de los transportadores de nucleótidos azúcares.

Ubicación y fono: Avda.República # 217, Piso 1 Centro de Biotecnologia Vegetal; (56-2) 6618628

Correo electrónico: aorellana@unab.cl

 

 

Líneas de Investigación

- Síntesis de Polisacáridos de pared celular en el Golgi: Contribución de los transportadores de nucleótidos azúcares
- Estrés en RE en plantas
- Genómica y Proteómica en frutales

Publicaciones

50. Ding, Z., Wang, B., Moreno, I., Duplá Ková, N., Simon, S., Carraro, N., Reemmer, J., Pě Nčí K, A., Chen, X., Tejos, R., SkÅpa, P., Pollmann, S., Mravec, J., Petrá Š Ek, J., ZaÅimalova, E., Honys, D., Rolčí K, J., Murphy, A., Orellana, A., Geisler, M. & Friml, J. (2012), “ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis”, Nature Communications, vol. 3: 941.

49. Ordenes, V.R., Moreno, I., Maturana, D., Norambuena, L., Trewavas, A.J. & Orellana, A. (2012), “In vivo analysis of the calcium signature in the plant Golgi apparatus reveals unique dynamics”, Cell calcium, 52: 397-404.

48. Balic, I., Moreno, A., Sanhueza, D., Huerta, C., Orellana, A., Defilippi, B.G. & Campos-Vargas, R. (2012), “Molecular and physiological study of postharvest rachis browning of table grape cv Red Globe”, Postharvest Biology and Technology, 72: 47-56.

47. Chiu, T.-., Christiansen, K., Moreno, I., Lao, J., Loqué, D., Orellana, A., Heazlewood, J.L., Clark, G. & Roux, S.J. (2012), “AtAPY1 and AtAPY2 function as golgi-localized nucleoside diphosphatases in arabidopsis thaliana”, Plant and Cell Physiology, 53: 1913-1925.

46. Handford, M., Rodríguez-Furlán, C., Marchant, L., Segura, M., Gómez, D., Alvarez-Buylla, E., Xiong, G.-., Pauly, M. & Orellana, A. (2012), “Arabidopsis thaliana AtUTr7 encodes a golgi-localized UDP-glucose/UDP- galactose transporter that affects lateral root emergence”, Molecular Plant, 5: 1263-1280.

45. Moreno, A.A., Mukhtar, M.S., Blanco, F., Boatwright, J.L., Moreno, I., Jordan, M.R., Chen, Y., Brandizzi, F., Dong, X., Orellana, A. & Pajerowska-Mukhtar, K.M. (2012), “IRE1/bZIP60-mediated unfolded protein response plays distinct roles in plant immunity and abiotic stress responses”, PLoS ONE, 7: e31944.

44. Nilo P., R., Campos-Vargas, R. & Orellana, A. (2012), “Assessment of Prunus persica fruit softening using a proteomics approach”, Journal of Proteomics, 75: 1618-1638.

43. Moreno, A.A., Orellana, A. (2011) The Physiological role of the Unfolded Protein Response in Plants” Biol Res 44: 75-80

42. Moreno, A. and Orellana, A. (2011) The physiological role of the unfolded protein response in plants. Biol. Res., 44: 75-80

41. Wulff-Zottele,C., Gatzke, N., Kopka, J., Orellana, A., Hoefgen, R., Fisahn, J., Hesse, H. (2010) “Photosynthesis and metabolism interact during acclimation of Arabidopsis thaliana to high irradiance and sulfur depletion” Plant Cell Environ 33: 1974-1988.

40. Nilo R, Saffie C, Lilley K, Baeza-Yates R, Cambiazo V, Campos-Vargas R, Gonzalez M,. Meisel LA, Retamales J, Silva H and Orellana A (2010) “Proteomic analysis of peach fruit mesocarp softening and chilling injury using difference gel electrophoresis (DIGE)” BMC Genomics 11: 43.

39. Reyes F, León G, Donoso M, Brandizzi F, Weber A, Orellana A (2010) The nucleotide sugar transporters AtUTr1 and AtUTr3 are required for the incorporation of UDP-glucose into the endoplasmic reticulum and are essential for pollen development and needed for embryo sac progress in Arabidopsis thaliana. Plant J 61: 423-435.

38. Vizoso P, Meisel LA, Tittarelli A, Latorre M, Saba J, Caroca R, Maldonado J, Cambiazo V, Campos-Vargas R, Gonzalez M, Orellana A, Silva H (2009) Comparative EST transcript profiling of peach fruits under different post-harvest conditions reveals candidate genes associated with peach fruit quality. BMC Genomics 10: 423.

37. Reyes F. and Orellana A. (2008) Golgi transporters: opening the gate to cell wall polysaccharide biosynthesis. Curr Op Plant Biol 11: 244-251.

36. Moreno I, Norambuena L, Maturana D, Toro M, Vergara C, Orellana A, Zurita-Silva A, Ordenes VR. (2008) AtHMA1 is a thapsigargin sensitive Ca2+/heavy metal pump. J Biol Chem 283: 9633-9641.

35. González-Agüero, M., Pavez, L., Ibáñez, F., Pacheco, I., Campos-Vargas, R., Meisel, L.,Orellana, A. Retamales, J., Silva, H., González, M. and Cambiazo, V. (2008) Identification of woolliness response genes in peach fruit after postharvest treatments J Exp Bot 59: 1973-1986.

34. Ibar, C. and Orellana, A (2007) “The Import of S-adenosyl Methionine into the Golgi Apparatus Is Required for the Methylation of Homogalacturonan” Plant Physiol. 145: 504-512.

33. De Pino, V., Borán, M. Norambuena, L., González, G., Reyes, F., Orellana, A. and Moreno, S.(2007) “Complex formation regulates the glycosylation of the reversibly glycosylated polypeptide” Planta 226:335-345.

32. Reyes F, Marchant L, Norambuena L, Nilo R, Silva H and Orellana A. (2006) AtUTr1, a UDP-glucose/UDP-galactose transporter from Arabidopsis thaliana, is located in the endoplasmic reticulum and up-regulated by the unfolded protein response. J Biol Chem. 281: 9145-9151.

31. Latorre, M., Silva, H., Saba, J., Guziolowski, C., Vizoso, P., Martinez, V., Maldonado, J., Morales, A., Caroca, R., Baeza-Yates, R., Cambiazo, V., Campos- Vargas, R., Gonzalez, M.,Orellana, A., Retamales, J., and Meisel, L. 2006. JUICE: a data management system which facilitates the analysis of large volumes of information in a EST project workflow. BMC Bioinformatics. 7:513.

30. Campos-Vargas, R., Becerra, O., Baeza-Yates, R., Cambiazo, V., González, M., Meisel, L., Orellana, A., Retamales, J., Silva, H., Defilippi, B.G. (2006) “Seasonal effects on the development of chilling injury development in peach (Prunus persica [L.] Batsch) cv. O`Henry” Scientia Horticulturae 110: 79-83.

29. Handford, M., Rodriguez-Furlan, C and Orellana, A. (2006) “Nucleotide-sugar transporters: Structure, function and roles in vivo” Braz J Med Biol Res. 39:1149-1158.

28. Norambuena L, Nilo R, Handford M, Reyes F, Marchant L, Meisel L and Orellana A.(2005) “AtUTr2 is an Arabidopsis thaliana nucleotide sugar transporter located in the Golgi apparatus capable of transporting UDP-galactose” Planta 222: 521-529.

27. Orellana A (2005) “Biosynthesis of non-cellulosic polysaccharides in the Golgi apparatus. Topological considerations”. Plant Biosystem 139: 42-45.

26. Meisel L, Fonseca B, Gonzalez S, Baeza-Yates R, Cambiazo C, Campos R, Gonzalez M,Orellana A, Retamales J and Silva H. (2005) “A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses” Biol Res 38: 83-88

25. Norambuena L, Marchant L, Berninsone P, Hirschberg CB, Silva H, Orellana A. (2002). “Transport of UDP-galactose in plants: Identification and functional characterization of AtUTr1, an Arabidopsis thaliana UDP-galactose/UDP-glucose transporter”. J Biol Chem 277: 32923-32929

24. Ordenes, VR. Reyes FC, Wolff D, and Orellana, A. (2002) “A Thapsigargin-Sensitive Ca2+ Pump Is Present in the Pea Golgi Apparatus Membrane”. Plant Physiol 129: 1820-1828.

23. Mura, C., Becker M.I., Orellana, A., and Wolff, D. (2002) “Immunopurification of Golgi vesicles by magnetic sorting” J. of Immunological Methods 260:263-271.

22. Baldwin, TC, MG Handford, M Yuseff, Orellana, A. and P. Dupree (2001) “Identification and characterization of GONST1, a Golgi-localized GDP-mannose transporter in Arabidopsis.” Plant Cell, 13, 2283-2295.

21. Sterling, J., Quigley, H.F., Orellana, A. and Mohnen, D. (2001). “The catalytic site of the pectin biosynthetic enzyme 1,4-galacturonosyltransferase (GalAT) is located in the lumen of the Golgi” Plant Physiol. 127: 360-371.

20. Wulff, C., Norambuena L. and Orellana, A. “GDP-Fucose incorporation into the Golgi during xloglucan biosynthesis requires the activity of a transporter- like protein other than the UDP-Glucose transporter” Plant Physiology 122: 867-878 (2000).

19. Rojas P, Surroca A, Orellana A, Wolff D (2000) Kinetic characterization of calcium uptake by the rat liver Golgi apparatus. Cell Biol Int 24:229-233.

18. Perrin,P., DeRocher,A., Bar-Peled,M., Zeng,W., Norambuena,L., Orellana,A., Raikhel,N. and Keegstra,K. “Xyloglucan Fucosyltransferase,an Enzyme Involved in Plant Cell Wall Biosynthesis” Science , 284: 1976-1979, (1999)

17. Orellana, A. and Mohnen, D. “Enzymatic Synthesis and Purification of [3H]Uridine Diphosphate Galacturonic Acid for Use in Studying Golgi-Localized Transporters” Analytical Biochemistry, 272: 224-231, (1999)

16. Chrispeels,M.; Holuigue,L.; Latorre,R.; Luan,S.; Orellana,A.; Peña-Cortés,H.; Raikhel,N.; Ronald,P.; Trewavas,A. “Signal Transduction networks and the Biology of Plant Cells” Biological Research, 32: 35-60, (1999)

15. Neckelmann,G. and Orellana, A. “Metabolism of UDP-glucose in Golgi Vesicles from Pea Stems (Pisum sativum)”. Plant Physiology 117: 1007-1014, (1998).

14. Arredondo, M.; Orellana, A.; Gárate, M. and Nuñez. M.T. “Intracellular Iron Regulates Iron Absorption and IRP in Intestinal Epithelial (Caco-2) Cells” Am. J. Physiol. 273, G275-G280, (1997).

13. Orellana, A.; Neckelmann,G., Muñoz,P. and Norambuena,L. “Topography and Function of Golgi UDPase from pea stems (Pisum sativum)” Plant Physiology 114: 99-107, (1997).

12. Muñoz,P.; Norambuena,L. and Orellana,A. “UDP-glucose Transport into the Lumen of Golgi Derived Vesicles from Pea Stems (Pisum sativum) is required for Polisaccharide Biosynthesis” Plant Physiology 112: 1585-1594, (1996).

11. Bronfman,M.; Nuñez,L.; Orellana,A.; Burzio,V. and Hidalgo,P. “Peroxisome Proliferators and Signal Transduction” Ann. N.Y. Acad. Sci. 804: 403-412, (1996).

10. Orellana,A., Hirschberg,C.B., Wei,Z., Swiedler,S.J. and Ishihara, M. “Molecular cloning and expression of a glycosaminoglycan N-acetylglucosaminyl N- deacetylase/N-sulfotransferase from a heparin-producing cell line” J. Biol. Chem. 269: 2270-2276, (1994).

09. Ishihara,M., Guo,Y., Wei,Z., Yang,Z., Swiedler,S.J., Orellana, A. and Hirschberg,C.B. “Regulation of biosynthesis of the basic fibroblast growth factor binding domains of heparan sulfate by heparan sulfate-N-deacetylase/N-sulfotransferase expression” J. Biol. Chem. 268: 20091- 20095, (1993).

08. Orellana,A., Holuigue,L., Hidalgo,P., Faundez,V., Gonzalez,A. and Bronfman,M. “Ciprofibrate, a carcinogenic peroxisome proliferator, increases the phosphorylation of epidermal-growth-factor receptor in isolated rat hepatocytes”. Eur. J. Biochem. 215: 903-906, (1993).

07. Wei,Z., Swiedler,S.J., Ishihara,M., Orellana,A. and Hirchberg,C.B. “A single protein catalyzes both N-deacetylation and N-sulfation during the biosynthesis of heparan sulfate” Proc. Natl. Acad. Sci. USA. 90: 3885-3888, (1993).

06. Hashimoto,Y., Orellana,A., Gil,G. and Hirschberg,C.B. “Molecular cloning and expression of rat liver N-heparan sulfate sulfotransferase”. J. Biol. Chem. 267: 15744-15750, (1992).

05. Bronfman,M., Morales,M.N., Amigo,L., Orellana,A., Nuñez,L., Cárdenas,L, and Hidalgo,P. “Hypolipidemic drugs are activated to acyl-coenzyme A esters in isolated hepatocytes”. Biochemical J. 284: 289-295, (1992).

04. Orellana, A., Kawada,M.E., Morales,M.N., Vargas,L. and Bronfman,M. “Induction of peroxisomal fatty acyl-coenzyme A oxidase and total carnitine acetyl- coenzyme A transferase in primary cultures of rat hepatocytes by garlic extracts”. Toxicology Lett. 60: 11-17, (1992).

03. Orellana,A., Hidalgo,P., Morales,M.N., Mezzano,D. and Bronfman,M. “Palmitoyl-CoA and the Acyl-CoA thioester of the carcinogenic peroxisome proliferator ciprofibrate potentiate diacylglicerol-activated protein kinase C by decreasing the phosphatidylserine requirement of the enzyme” Eur. J. Biochem. 190: 57-61, (1990).

02. Bronfman,M., Orellana,A., Morales,M.N., Bieri,F., Waechter,F., Staubli,W. and Bentley,P. “Potentiation of diacylglicerol activated protein kinase C by acyl-Coenzyme A thioesters of hypolipidemic drugs” Biochem. Biophys. Res. Commun. 159: 1026-1031, (1989).

01. Bronfman, M., Morales,M.N., and Orellana,A. “Diacylglycerol activation of protein kinase C is modulated by long chain acyl-CoAs” Biochem. Biophys. Res. Commun. 152: 987-992, (1988).

Publicaciones (No-ISI)

03. Bronfman,M.; Nuñez,L.; Orellana,A.; Burzio,V. and Hidalgo,P. (1996) “Peroxisome Proliferators and Signal Transduction” Ann. N.Y. Acad. Sci. 804: 403-412.

02. Seibert, E., González,S., Luchsinger, L., Orellana, A., and Bender R.J. (2006) “Efecto del almacenaje refrigerado sobre la calidad y el desarrollo de daños por frío en duraznos ‘Sweet September’ Rev. Iber. Tecnología Postcosecha 7: 131- 141.

01. Seibert, E.; Gonzalez, S.; Orellana, A.; Luchsinger, L. and Bender, R.J. (2008) Efecto del acondicionado previo al almacenaje refrigerado sobre la calidad de ciruelas ‘Constanza’. Bragantia, 67: 233-242.