Dr. Martin Montecino Leonard

Dr. Martin Montecino Leonard

Profesor Titular y Director, Centro de Investigaciones Biomédicas y Director de Programa de Doctorado en Biociencias Moleculares, Universidad Andrés Bello

Profesor Asociado Adjunto, Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.

Bioquímico, Universidad de Concepción 1988

Doctor en Ciencias Biomédicas con mención en Biología Celular y Molecular, University of Massachusetts, 1996

Magister en Ciencias con mención en Bioquímica, Universidad de Concepción, 1991

Área de especialización: Biología Celular y Molecular

Ubicación y fono: Laboratorio de Regulación Génica, Centro de Investigaciones Biomédicas, República 239 (Interior). Teléfono: 56-2-7703235.

correo electronico: mmontecino@unab.cl

Línea de investigación

Mecanismos que regulan la expresión de genes durante la diferenciación celular.

Publicaciones

2010

Montecino, M.A., Lian, J.B., Stein, J.L., Stein, G.S., van Wijnen, A.J. and Cruzat, F. (2010) Biological and molecular effects of vitamin D on bone. In “Vitamin D: Physiology, Molecular Biology and Clinical Applications”. Edited by Holick, M.F. The Humana Press, NJ. In press.

Zaidi, S.K., Medina, R.F., Pockwinse, S.M., Bakshi, R. Kota, K.P., Ali, S.A., Young, D. W., Nickerson, J.A., Javed, A., Montecino, M., van Wijnen, A., Lian, J.B., Stein, J.L. and Stein, G.S. (2010) Subnuclear localization and intranuclear trafficking of transcription factors. In “Transcription Factors: Methods and Protocols, Methods in Molecular Biology”, Edited by Higgins, P.J., Springer Science and Buissness Media,Vol 647, 77-93.

Stein, G.S., Stein, J.L., van Wijnen, A.J., Lian, J.B., Montecino, M., Croce, C., Choi, J. Y., Ali, S.A., Pande, S., Hassan, M.Q., Zaidi, S.K., Young, D.W. (2010) Transcription factor-mediated epigenetic regulation of cell growth and phenotype for biological control and cancer. Adv. Enzyme Regul. 50: 160-167.

Arriagada, G., Paredes, R., Olate, J., van Zundert, B., Lian, J., Stein, G., Stein, J. and Montecino, M. (2010) 1,25-dihydroxy vitamin D3 induces nuclear matrix association of1,25-dihydroxy vitamin D3 receptor in osteoblasts independently of its ability to bind DNA. J. Cell. Physiol. 222: 336-346.

Arriagada, G., Henríquez, B., Moena, D., Merino, P., Ruiz-Tagle, C., Lian, J.B., Stein, G.S., Stein, J.L. and Montecino, M. (2010) Recruitment and subnuclear distribution of the regulatory machinery during 1,25-dihydroxy vitamin D3-mediated transcriptional upregulation in osteoblasts. J. Ster. Biochem. Mol. Biol. 121: 156-158.

Sepúlveda, F., Bustos, F., Inostroza, E., Zuñiga, F., Neve, R., Montecino, M., van Zundert, B. (2010) NR2B-NMDA receptors regulate dendritic branching via interaction with CaMKII and RasGRF1. J. Neurophysiol. Epub Jan 27.

Marcellini, S., Bruna, C., Henriquez J.P., Albistur, M., Reyes, A. E., Barriga, E.H., Henriquez, B. and Montecino, M. (2010) Evolution of the interaction between Runx2 and VDR, two transcription factors involved in osteoblastogenesis. BMC Evol. Biol. 10: 78.

Gordon, J.A.R., Hassan, M.Q., Saini, S., Montecino, M., van Wijnen, A.J., Stein, G.S., Stein, J. and Lian, J.B. (2010) Pbx1 represses osteoblastogenesis by blocking Hoxa10-mediated recruitment of chromatin remodelling factors. Mol. Cell. Biol. 30: 3531-3541.

Zaidi, S.K., Young, D.W., Montecino, M., Lian, J.B., van Wijnen, A.J., Stein, J.L. and Stein, G.S. (2010) Mitotic bookmarking of genes: a novel dimension to epigenetic control. Nat. Rev. Genet., 11: 583-589.

Bakshi, R., Hassan, M.Q., Pratap, J., Lian, J.B., Montecino, M., van Wijnen, A.J., Stein, J.L., Imbalzano, A.N. and Stein, G.S. (2010) Human SWI/SNF complex associates with RUNx1 to control transcription of hematopoietic target genes. J. Cell. Physiol., 225: 569-576.

Zaidi, S.K., Young, D.W., Montecino, M., Lian, J.B., van Wijnen, A.J., Stein, J.L. and  Stein, G.S. (2010) Architectural epigenetics: mitotic retention of transcriptional regulatory information. Mol. Cell. Biol. Epub August 9th.

Stein, G.S., van Wijnen, A.J., Imbalzano, A.N., Montecino, M., Lian, J.B., Nickerson, J. A., Stein, J.L. (2010) Architectural genetic and epigenetic control of regulatory networks: compartmentalizing machinery for transcription and chromatin remodelling in nuclear microenvironments. Crit. Rev. Euk. Gene Exp., In press.

2009

Stein, G., Zaidi, S., Stein, J., Lian, J., van Wijnen, A., Montecino, M., Young, D., Javed, A., Pratap, J., Choi, J., Ali, S., Pande, S. and Hassan, M. (2009) Organization, integration, and assembly of genetic and epigenetic regulatory machinery in nuclear microenvironments: implications for biological control in cancer. Ann. New York Acad. Sci., 1155: 4-14.

Stein, G., Zaidi, S., Stein, J., Lian, J., van Wijnen, A., Montecino, M., Young, D., Javed, A., Pratap, J., Choi, J., Ali, S., Pande, S. and Hassan, M. (2009) Transcription-factor-mediated epigenetic control of cell fate and lineage commitment. Biochem. Cell Biol., 87: 1-6.

Zhang, Y., Hassan, M., Xie, R., Hawse, J., Spelsberg, T., Montecino, M., Stein, J., Lian, J., van Wijnen, A. and Stein, G. (2009) Co-stimulation of the bone-related Runx2 P1 promoter in mesenchymal cells by SP1 and ETS transcription factors at polymorphic purine-rich DNA sequences (Y-repeats). J.  Biol. Chem. 284: 3125-35.

Pande, S., Ali, S., Dowdy, C., Zaidi, S., Ito, K., Ito, Y., Montecino, M., Lian, J., Stein, J., van Wijnen, A. and Stein, G. (2009) Subnuclear targeting of the Runx3 tumor suppressor and its epigenetic association with mitotic chromosomes. J. Cell. Physiol. 218: 473-9.

Cruzat, F., Henriquez, B., Villagra, Gutierrez, S., A., Lian, J. B., Stein, J. L., van Wijnen, A., Stein, G. and Montecino, M. (2009) SWI/SNF-independent nuclease hypersensitivity and increased histone acetylation at the P1 promoter accompany active transcription of the bone master gene Runx2. Biochemistry 48: 7287-7295.

Arrázola, M., Varela-Nallar, L., Cruzat, F., Pavez, L., Assar, R., Aravena, A., Toledo, E., Colombres, M., Gonzalez, M., Montecino, M., Maass, A., Martinez, S. and Inestrosa, N. (2009) Calcium/calmodulin-dependent kinase IV is a target gene of the Wnt/beta-catenin pathway. J. Cell. Physiol. 221: 658-667.

2008

Ali, S., Zaidi, S., Dacwag, C., Salma, N., Young, D., Shakoori, A., Montecino, M., Lian, J., van Wijnen, A., Imbalzano, A., Stein, G. and Stein, J. (2008) Phenotypic transcription factors epigenetically mediate cell growth control. Proc. Natl. Acad. Sci. USA. 105: 6632-6637.

Van der Deen, M., Hassan, M., Pratap, J., Teplyuk, N., Young, D., Javed, A., Zaidi, K., Lian, J., Montecino, M., Stein, J., Stein, G. and van Wijnen, A. (2008) Chromatin immunoprecipitation assays: application of ChIP-on-chip for defining dynamic transcriptional mechanisms in bone cells. Methods Mol. Biol. 455: 165-176.

Pockwinse, S., Zaidi, K., Medina, R., Bakshi, R., Kota, K., Ali, S., Young, D., Nickerson, J., Javed, A., Montecino, M., van Wijnen, A., Lian, J., Stein, J. and Stein, G. (2008) In situ organization of regulatory machinery. Methods Mol. Biol. 455: 239-259.

Stein, G., Zaidi, K., Stein, J., Lian, J., van Wijnen, A., Montecino, M., Young, D., Javed, A., Pratap, J., Choi, J., Ali, S., Pande, S., Hassan, M. (2008) Genetic and epigenetic regulation in nuclear microenvironments for biological control of cancer. J. Cell. Biochem., 104: 2016-2026.

Hassan, M., Saini, S., Gordon, J., van Wijnen, A., Montecino, M., Stein, J., Stein, G., Lian, J.  (2008) Molecular switches involving homeodomain proteins, HOXA10 and RUNX2 regulate osteoblastogenesis. Cells Tiss. Org., 189: 122-125.

Stuardo, M., Martinez, M., Hidalgo, K., Montecino, M., Javed, A., Lian, J., Stein, G., Stein, J. and Gutierrez, S. (2008) Altered chromatin modifications in AML/RUNX1 breakpoint regions involved in (8;21) translocation. J. Cell. Physiol. 8: 343-349.

Bakshi, R., Zaidi, K., Pande, S., Hassan, M., Young, D., Montecino, M., Lian, J., van Wijnen, A., Stein, J. and Stein, G. (2008) The leukemogenic t(8;21) fusion protein AML1-ETO controls rRNA genes and associates with nucleolar-organizing regions at mitotic chromosomes. J. Cell Sci. 121: 3981-3990.

Soto, X., Mayor, R., Torrejon, M., Montecino, M., Hinrichs, M.V. and Olate, J. (2008) Gq negatively egulates the Wnt--catenin pathway and dorsal embryonic Xenopus laevis development. J. Cell. Physiol. 214: 483-490.

Montecino, M., Stein, G.S., Stein, J.L., Lian, J. B., van Wijnen, A.J., Marcellini, S., Cruzat, F. and Arriagada, G. (2008) Vitamin D control of gene expression: temporal and spatial parameters for organization of the regulatory machinery. Crit. Rev. Eukaryot. Gene Expr. 18: 163-172.

Carvallo, L., Henriquez, B., Paredes, R., Olate, J., Onate, S., van Wijnen, A.J., Lian, J. B., Stein, G.S., Stein, J.L. and Montecino, M. (2008) 1,25-dihydroxyvitamin D3-enhanced transcription of the bone-specific osteocalcin gene involves preferential recruitment of the coactivator SRC-1. J. Cell. Physiol., 214: 740-749.

2007

Young, D., Hassan, M., Pratap, J., Galindo, M., Zaidi, S-K., Lee, S., Yang, X., Xie, R., Underwood, J., Furcinitti, P., Imbalzano, A., Penman, S., Nickerson, J., Montecino, M., Lian, J., Stein, J., van Wijnen, A. and Stein, G. (2007) Mitotic occupancy and lineage-specific transcriptional control of ribosomal RNA genes by the Runx2 transcription factor. Nature, 445: 442-446.

Martinez, S., Pasten, P., Suarez, K., Garcia, A., Nualart, F., Montecino, M., Hinrichs, M.V., Olate, J. (2007) Classical Xenopus laevis progesterone receptor associates to the plasma membrane through its ligand-binding domain. J. Cell. Physiol. 211: 560-567.

Carvallo, L., Henríquez, B., Olate, J., van Wijnen, A.J., Lian, J. B., Stein, G.S., Onate, S., Stein, J.L. and Montecino, M. (2007) The 1,25-dihydroxyvitamin D3 receptor preferentially recruits the coactivator SRC-1 during up-regulation of the osteocalcin gene. J. Ster. Biochem. Mol. Biol. 103(3-5): 420-424.

Arriagada, G., Paredes, R., Olate, J., van Wijnen, A. J., Lian, J.B., Stein, G.S., Stein, J. L., Onate, S. and Montecino, M. (2007) Phosphorylation at serine 208 of the 1,25-dihydroxyvitamin D3 receptor modulates the interaction with transcriptional coactivators. J. Ster. Biochem. Mol. Biol. 103(3-5): 425-429.

Montecino, M., Stein, G., Cruzat, F., Marcellini, S., Stein, J., Lian, J., van Wijnen, A. and Arriagada, G. (2007) An architectural perspective of vitamin D responsiveness. Arch. Biochem. Biophys. 41: 272-278.

Bruna, C., Arriagada, G., Lian, J.B., Stein, G. S., Bunster, M., Martínez-Oyanedel, J. and Montecino, M. (2007) Crystallization and preliminary X-ray analysis of a domain in the Runx2 transcription factor that interacts with the 1,25-dihydroxy vitamin D3 receptor. J. Cell. Biochem. 101: 785-789.

Gutierrez, J., Paredes, R., Hill, D. A., van Wijnen, A.J., Lian, J.L., Stein, G.S., Stein, J. L., Imbalzano, A.N., Montecino, M. (2007) Chromatin remodeling by SWI/SNF results in nucleosome mobilization to preferential positions in the rat osteocalcin gene promoter. J. Biol. Chem. 282: 9445-9457.

Young, D., Hassan, M., Yang, X. Q., J., Galindo, M., Javed, A., Zaidi, S-K., Lee, S., Furcinitti, P., Lapointe, D., Montecino, M., Lian, J., Stein, J., van Wijnen, A. and Stein, G. (2007) Mitotic regulation of gene expression patterns by cell fate-determining transcription factor Runx2. Proc. Natl. Acad. Sci. USA. 104: 3189-3194.

Hassan, M.Q., Tare, R., Lee, S.H., Weiner, B., Montecino, M., van Wijnen, A., Stein, J. L., Stein, G.S. and Lian, J. (2007) HOXA10 controls osteoblastogenesis by directly activating bone regulatory and phenotypic genes. Mol. Cell. Biol. 27: 3337-3352.

Stein, G.S., Lian, J.B., van Wijnen, A.J., Stein, J.L., Javed, A., Montecino, M., Choi, J. Y., Vradii, D., Zaidi, S.K., Pratap, J. and Young, D. (2007) Organization of transcriptional regulatory machinery in nuclear microenvironments: implication for biological control and cancer. Adv. Enzyme Reg. 47: 242-250.

Zaidi, S.K., Young, D.W., Javed, A., Pratap, J., Montecino, M., van Wijnen, A.J., Lian, J.B., Stein, J.L., Stein, G.S. (2007) Nuclear microenvironment in biological control and cancer. Nat. Rev. Cancer 7: 454-463.

Montecino, M., Stein, J.L., Stein, G.S., Lian, J.B., van Wijnen, A., Cruzat, F., Gutierrez, S., Olate, J., Marcellini, M. and Gutierrez, J.L. (2007) Nucleosome organization and targeting of ATP-dependent chromatin remodeling complexes: contributions of the DNA sequence. Biochem. Cell Biol. 85: 419-425.

Romo, X., Pasten, P., Martinez, S., Soto, X., Lara, P., Ramirez de Arellano, M. A., Torrejon, M., Montecino, M., Hinrichs, M.V. and Olate, J. (2007) xRic-8 is aGEF and participates in maintaining meiotic arrest in Xenopus laevis oocytes. J. Cell. Physiol., 241: 673-680.

2006

Martinez, S., Grandy, R., Pasten, P., Montecinos, H., Montecino, M., Olate, J. and Hinrichs, M.V. (2006) Plasma membrane destination of the classical Xenopus laevis progesterone receptor accelerates progesterone-induced oocyte maturation. J. Cell. Biochem. 99: 853-859.

Villagra, A., Cruzat, F., Carvallo, L., Olate, J., van Wijnen, A., Stein, G., Lian, J., Stein, J., Imbalzano, A. and Montecino, M. (2006) Chromatin remodeling and transcriptional activity of the bone-specific osteocalcin gene require C/EBP-dependent recruitment of SWI/SNF activity. J. Biol. Chem. 281: 22695-22706.

Bravo, S., Paredes, R., Izaurieta, P., Lian, J., Stein, J., Stein, G., Hinrichs, M.V., Olate, J., Aguayo, L. and Montecino, M. (2006) The classic receptor for 1,25-dihydroxyvitamin D3 is required for non genomic actions of 1,25-dihydroxyvitamin D3 in osteoblastic cells. J. Cell. Biochem. 99: 995-1000.

Stein, G.S., van Wijnen, A.J., Stein, J.L., Lian, J.B., Montecino, M., Zaidi, S.K. and Braastad, C. (2006) An architectural perspective of cell-cycle control at the G1/S phase cell-cycle transition. J. Cell. Physiol. 209: 706-710.

Lian, J.B., Stein, G.S., Javed, A., van Wijnen, A.J., Stein, J.L., Montecino, M., Hassan, M.Q., Gaur, T, Lengner, C.J. and Young, D.W. (2006) Networks and hubs for the transcriptional control of osteoblastogenesis. Rev. Endocr. Metab. Disord. 7: 1-16.

2005

-Guzmán, L., Romo, X., Grandy, R., Soto, X., Montecino, M., Hinrichs, M.V., Olate, J. (2005) A G stimulated adenylyl cyclase is involved in Xenopus laevis oocyte maturation. J. Cell. Physiol., 202: 223-229.

-Zaidi, S., Young, D., Choi, J., Pratap, J., Javed, A., Montecino, M., Stein, J., van Wijnen, A., Lian, J., Stein, G. (2005) The dynamic organization of gene-regulatory machinery in nuclear microenviroments. EMBO Rep., 6: 128-33.

-Vradij, D., Wagner, S., Doan, D. N., Nickerson, J., Montecino, M., Lian, J. B., Stein, J. L., van Wijnen, A. J., Imbalzano, A. N., Stein, G. S. (2005) Brg1, the ATPase subunit of the SWI/SNF chromatin remodeling complex is required for myeloid differentiation to granulocytes. J Cell Physiol, 206, 112-118.

-Stein, G. S., Lian, J. B., Stein, J. L., van Wijnen, A. J., Javed, A., Montecino, M., Zaidi, S. K., Young, D., Choi, J., Pratap, J. (2005) Combinatorial organization of the transcriptional regulatory machinery in biological control and cancer. Adv Enzyme Regul. 45: 136-154.