Dra. Gloria Arriagada

Dra. Gloria Arriagada

Profesor Asistente

Bioquímico, Universidad de Concepción, 2004

Doctor Ciencias biológicas, Universidad de Concepción, 2007

Area de especialización: Retrovirus, interaccion virus-hospedero

Ubicación y Fono: Facultad de Ciencias Biologicas-Sede Viña del Mar, Quillota 910 4ºPiso torre C. Teléfono 32-2845931. Laboratorio de Virología Molecular, idem dirección BIO415, teléfono 32-2845948.

Correo electrónico: gloria.arriagada@unab.cl

Área de investigación:

This is a very exciting time for the research in the field of retroviruses. Even though there is a great knowledge about the mechanisms used by retroviruses to infect cells and cause diseases, we still have much more to learn. As an example, how the retrovirus traffic from the cell membrane to the nucleus is not completely clear.

Our work model, Murine Leukemia Virus (MLV), has been successfully used in the past to identified host factors that affect retroviral infection; including infection by the human immunodeficiency virus (HIV), the causative agent of one of the biggest pandemics of our time in the human population, AIDS.

The understanding of the strategies used by MLV to traffic from the cell membrane to the nucleus will provide important clues to design or develop new antiviral drugs that can specifically disrupt intracellular viral trafficking, not only of MLV but other retroviruses that affect humans and animals, such as HIV, feline immunodefiency virus (FIV) and human T-lymphotropic virus (HTLV).

Publicaciones:

2013

Lukic, Z., Goff, S.P., Campbell, E.M. and Arriagada, G. (2013) Role of SUMO-1 and SUMO interacting motifs in rhesus TRIM5alpha-mediated restriction. Retrovirology  10(1):10

Arriagada, G., Muntean, L.N. and Goff, S.P. (2013) SUMO-interacting Motifs of Human TRIM5α are Important for Antiviral Activity. PLoS Pathog 7(4): e1002019

Arriagada, G., Henriquez, B., Moena, D., Merino, P., Ruiz-Tagle, C., Lian, J.B., Stein, G.S.,  Stein, J.L. and Montecino, M. (2013) Recruitment and subnuclear distribution of the regulatory machinery during 1alpha,25-dihydroxy vitamin D(3)-mediated transcriptional upregulation in osteoblasts. J Steroid Biochem Mol Biol. 121(1-2):156-8

2009

Arriagada, G., Paredes., R., van Wijnen, A.J., Lian, J.B., van Zundert, B., Stein, G.S., Stein, J.L. and Montecino, M. (2009) 1alpha,25-dihydroxy vitamin D(3) induces nuclear matrix association of the 1alpha,25-dihydroxy vitamin D(3) receptor in osteoblasts independently of its ability to bind DNA. J Cell Physiol. 222(2):336-346.

Valente, S.T., Gilmartin, G.M., Venkatarama, K., Arriagada, G. and Goff, S.P. (2009) HIV-1 mRNA 3′ end processing is distinctively regulated by eIF3f, CDK11, and splice factor 9G8. Mol Cell. 2009 Oct 23;36(2):279-89.

2008

Montecino, M., Stein, G.S., Stein, J.L., Lian, J.B., van Wijnen, A.J., Carvallo, L., Marcellini, S., Cruzat, F. and Arriagada, G. (2008) Vitamin D control of gene expression: temporal and spatial parameters for organization of the regulatory machinery. Crit Rev Eukaryot Gene Expr. 18(2):163-72.

2007

Arriagada, G., Paredes, R., Olate, J., van Wijnen, A., Lian, J.B., Stein, G.S., Stein, J.L., Onate, S. and Montecino, M. (2007) Phosphorylation at serine 208 of the 1alpha,25-dihydroxy Vitamin D3 receptor modulates the interaction with transcriptional coactivators.  J Steroid Biochem Mol Biol. 103(3-5):425-9.

Montecino, M, Stein, GS, Cruzat, F, Marcellini, S, Stein, JL, Lian, JB, van Wijnen, AJ, and Arriagada, G. (2007) An architectural perspective of vitamin D responsiveness. Arch Biochem Biophys 460(2):293-9.

Bruna, C., Arriagada, G., Lian, J.B., Stein, G.S., Bunster, M., Martinez-Oyanedel, J., and Montecino, M. (2007) Crystallization and preliminary X-ray analysis of a domain in the Runx2 transcription factor that interacts with the 1alpha,25 dihydroxy vitamin D3 receptor. J Cell Biochem. 101(3):785-9.

2004

Paredes, R., Arriagada, G., Cruzat, F., Villagra, A., Olate, J., Zaidi, K., van Wijnen, A., Lian, J.B., Stein, G.S., Stein, J.L. and Montecino, M. (2004) Bone-specific transcription factor Runx2 interacts with the 1alpha,25-dihydroxyvitamin D3 receptor to up-regulate rat osteocalcin gene expression in osteoblastic cells. Mol Cell Biol. 2004 Oct;24(20):8847-61.

Paredes, R., Arriagada, G., Cruzat, F., Olate, J., Van Wijnen, A., Lian, J., Stein, G., Stein, J., and Montecino, M. (2004) The Runx2 transcription factor plays a key role in the 1alpha,25-dihydroxy Vitamin D3-dependent upregulation of the rat osteocalcin (OC) gene expression in osteoblastic cells. J Steroid Biochem Mol Biol 89-90(1-5):269-71.

2003

Sierra, J., Villagra, A., Paredes, R., Cruzat, F., Gutierrez, S., Javed, A., Arriagada, G., Olate, J., Imschenetzky, M., Van Wijnen, A.J., Lian, J.B., Stein, G.S., Stein, J.L. and Montecino, M. (2003) Regulation of the bone-specific osteocalcin gene by p300 requires Runx2/Cbfa1 and the vitamin D3 receptor but not p300 intrinsic histone acetyltransferase activity. Mol Cell Biol. 23(9):3339-51